Detailed information of TA system TA048490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1994254..1995911
• Replicon: chromosome
• Accession: NZ_CP009159
• Organism: Actinobacillus suis ATCC 33415

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: K0FZC7
• Locus tag: ASU1_RS09195
• Protein ID: WP_015674102.1
• Coordinates: 1994580..1995911 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: K0G670
• Locus tag: ASU1_RS09190
• Protein ID: WP_015674101.1
• Coordinates: 1994254..1994583 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU1_RS09175	1990641..1991921	+	1281	WP_015674098.1	threonine synthase	-
ASU1_RS09180	1991999..1992337	+	339	WP_015674099.1	hypothetical protein	-
ASU1_RS09185	1992635..1993960	-	1326	WP_015674100.1	MFS transporter family glucose-6-phosphate receptor UhpC	-
ASU1_RS09190	1994254..1994583	-	330	WP_015674101.1	helix-turn-helix domain-containing protein	Antitoxin
ASU1_RS09195	1994580..1995911	-	1332	WP_015674102.1	type II toxin-antitoxin system HipA family toxin	Toxin
ASU1_RS11835	1996308..1997336	+	1029	Protein_1789	Abi family protein	-
ASU1_RS09210	1997493..1998530	-	1038	WP_015674105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-387)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51155.04 Da
• Isoelectric Point: 5.1273

>T48490 WP_015674102.1 NZ_CP009159:c1995911-1994580 [Actinobacillus suis ATCC 33415]
MSDISIKIKKLTLQTYVDNLWRDMAELYFNAQYELESMEYLTDYAVDFFGQANARSISINYPVNVFIDHADKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAVPEIQSERYFPLNVVQNRQSDFLEYANQTGAIVGG
ATGAGGEAPKLLLKRQDKQVWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
ERYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNVISASQFSNDFDNRHFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNRIWLSPIYDFAPMRADPEGIIRTTTWAKNGDNSQEFAGEYRFDKIADSLSDLIEPDILLRELR
MLATQLVDLKSRLTKRGVPKSILDFPAIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

>T48490 NZ_CP009159:c1995911-1994580 [Actinobacillus suis ATCC 33415]
ATGAGTGATATATCAATCAAAATTAAAAAACTAACATTGCAGACCTATGTTGATAATTTATGGCGAGATATGGCGGAGCT
TTATTTCAATGCTCAGTATGAATTAGAGAGCATGGAATATTTAACGGACTATGCCGTCGATTTTTTTGGGCAAGCGAATG
CCAGATCCATATCAATTAATTATCCTGTAAATGTGTTTATTGATCATGCTGATAAGCTGATGACTACACTTGCCGATATT
ATTCCTGCCGGAGCGAGTCGGCGTTATTGGCTGGAAGCTCTTGATATCAAGCACCTATCTATTTCTCATCAAAATTACTT
ATTACTAAAAAATGGCGCAATATCCCCTATCGGCAATGTTCGAGTTAAAGAGGCTGTGCCAGAAATACAAAGTGAGCGTT
ATTTTCCATTAAATGTGGTACAAAACAGGCAGAGTGATTTTTTAGAATATGCAAATCAGACAGGAGCTATTGTAGGCGGA
GCGACCGGGGCTGGGGGAGAAGCACCAAAGTTATTACTTAAACGCCAAGATAAACAAGTTTGGATTGATAATTTGCAATT
AGGTTTAGATTGCGATACGGCTTATTTAGTGAAATATCCTCGTGGGAATTATTCAGAGTTGGACTGTGATATTTTAAGAG
CTGAATATCATTATTATCATGAGCTGCGTGCATTAGGGTTTTCAACTATAGATACTGATCTAATGAAATTAGAAGAAGGG
GAACGTTATCCGTCTTTATGGCTACCTAGATTCGATATGAAGTTCAAAGATGGAAAATGGGAGCGATATGCTATGGAATC
GGTTTATTCTATGTTGCAAAAATATGGTGGAAATCTTGATCATGAGCAGACTATTCGCAACTTACTCAATGTTATTTCAG
CTTCGCAATTTTCTAATGATTTTGATAACCGGCATTTTGTTATTGAATGGGTAAAAAGGGATTTGTTGAATATTGCTTTT
GGAAATAGTGATAATCATGGCAGAAATACTGCATTTTTACGTGATGATAATCGTATTTGGCTATCCCCTATCTATGATTT
TGCTCCGATGCGGGCAGATCCGGAAGGCATTATCAGAACGACCACTTGGGCTAAAAATGGAGATAACTCTCAAGAGTTCG
CCGGAGAATATCGCTTTGATAAAATAGCTGACAGTTTATCTGACCTTATTGAACCAGATATATTGTTAAGAGAATTAAGA
ATGCTTGCGACCCAATTAGTAGATTTAAAATCTAGACTTACGAAGCGAGGTGTTCCGAAAAGCATTTTAGATTTTCCCGC
CATTGGTTTTGATTACTTACCTGAGAAGTTACAACGTTGGGGGCTGGTATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12364.46 Da
• Isoelectric Point: 10.6799

>AT48490 WP_015674101.1 NZ_CP009159:c1994583-1994254 [Actinobacillus suis ATCC 33415]
MTIQHKNASPTRSKVNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETINQVFKPMGLKLGLMPISKDLMDSFLK

Nucleotide

• Length: 330 bp

>AT48490 NZ_CP009159:c1994583-1994254 [Actinobacillus suis ATCC 33415]
ATGACGATTCAACATAAAAATGCCTCACCGACGCGCTCTAAAGTGAATGGCATTGAACGACAATTAATCCGTGAAACGAT
TATAGTACAGCTCTTAAAAGGTGAGATTAGCCAAGGGCAAGCACTGCGTCGATTAAGAGTGGAAGCATTGGGAATAAACC
AGCAAGATTATCTGAAATTAGCGAAAGTTTCTCGGCAAACTCTTTCTAATATCGAAAATGATAAAGGAAACTATAGTATA
GAAACGATTAATCAGGTTTTTAAACCGATGGGGCTAAAGCTAGGTCTTATGCCGATTTCAAAAGACTTGATGGATTCCTT
TCTAAAATAA

Structures

Toxin

• AlphaFold DB: K0FZC7

Antitoxin

• AlphaFold DB: K0G670