Detailed information of TA system TA048489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1930665..1931396
• Replicon: chromosome
• Accession: NZ_CP009159
• Organism: Actinobacillus suis ATCC 33415

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: K0FZ70
• Locus tag: ASU1_RS08860
• Protein ID: WP_015674046.1
• Coordinates: 1930665..1931132 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: K0G6P2
• Locus tag: ASU1_RS08865
• Protein ID: WP_015674047.1
• Coordinates: 1931136..1931396 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU1_RS08845	1926607..1927983	+	1377	WP_015674043.1	Trk system potassium transporter TrkA	-
ASU1_RS08850	1928196..1929590	+	1395	WP_015674044.1	class II fumarate hydratase	-
ASU1_RS08855	1929701..1930582	-	882	WP_015674045.1	50S ribosomal protein L11 methyltransferase	-
ASU1_RS08860	1930665..1931132	-	468	WP_015674046.1	GNAT family N-acetyltransferase	Toxin
ASU1_RS08865	1931136..1931396	-	261	WP_015674047.1	DUF1778 domain-containing protein	Antitoxin
ASU1_RS08870	1931517..1932977	-	1461	WP_015674048.1	metalloprotease TldD	-
ASU1_RS08875	1933107..1934366	+	1260	WP_015674049.1	tRNA lysidine(34) synthetase TilS	-
ASU1_RS08880	1934467..1934967	-	501	WP_015674050.1	thiol peroxidase	-
ASU1_RS08885	1935100..1935825	+	726	WP_015674051.1	1-acylglycerol-3-phosphate O-acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17157.14 Da
• Isoelectric Point: 8.9515

>T48489 WP_015674046.1 NZ_CP009159:c1931132-1930665 [Actinobacillus suis ATCC 33415]
MHAPELLSEQHIVQHFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSRQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T48489 NZ_CP009159:c1931132-1930665 [Actinobacillus suis ATCC 33415]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCAACATTTTCAATGCGGTGAGGTCGTACTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACTTTTGTTGTGTGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGTCGTCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGCTCAGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAGTCGAAGACGGTGGCGAACCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9826.17 Da
• Isoelectric Point: 4.4883

>AT48489 WP_015674047.1 NZ_CP009159:c1931396-1931136 [Actinobacillus suis ATCC 33415]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQTFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT48489 NZ_CP009159:c1931396-1931136 [Actinobacillus suis ATCC 33415]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGTATTTTAGGTAA
GACACGTTCTGACTTTATGTTAGAAACCGCATGCCAAGCTGCCCAAAATGTGATTTTAGATCGCACTGTTTTCCAATTAA
ATGAGCAGACTTTTGAGCAATTTAACCGATTGCTAGAACAACCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: K0FZ70

Antitoxin

• AlphaFold DB: K0G6P2