Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1228914..1229494 Replicon chromosome
Accession NZ_CP009158
Organism Glaesserella parasuis strain SH03

Toxin (Protein)


Gene name mazF Uniprot ID A0A143CFE3
Locus tag JL26_RS06150 Protein ID WP_005710599.1
Coordinates 1229150..1229494 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U4SAI4
Locus tag JL26_RS06145 Protein ID WP_005710601.1
Coordinates 1228914..1229153 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL26_RS06120 1224618..1225310 - 693 WP_010785974.1 tellurite resistance TerB family protein -
JL26_RS06125 1225372..1226220 - 849 WP_021110479.1 ABC transporter permease subunit -
JL26_RS06130 1226217..1227002 - 786 WP_015940139.1 ABC transporter permease subunit -
JL26_RS06135 1226992..1227741 - 750 WP_035494057.1 phosphonate ABC transporter ATP-binding protein -
JL26_RS06140 1227853..1228728 - 876 WP_005710603.1 putative selenate ABC transporter substrate-binding protein -
JL26_RS06145 1228914..1229153 + 240 WP_005710601.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JL26_RS06150 1229150..1229494 + 345 WP_005710599.1 endoribonuclease MazF Toxin
JL26_RS06155 1229500..1230168 + 669 WP_005710597.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
JL26_RS06160 1230181..1231572 + 1392 WP_005710595.1 MATE family efflux transporter -
JL26_RS06165 1231644..1233749 - 2106 WP_038514417.1 glycoside hydrolase family 2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1234430..1234720 290


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12830.81 Da        Isoelectric Point: 7.9277

>T48482 WP_005710599.1 NZ_CP009158:1229150-1229494 [Glaesserella parasuis]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPTIYNRQTGLLICCPLTTKVKGYPFEVNIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Download         Length: 345 bp

>T48482 NZ_CP009158:1229150-1229494 [Glaesserella parasuis]
ATGACTACTCAAGAGTATATCCCTGACGTTGGTGATATTATTTGGCTTGATTTTGATCCGCAAGCAGGACATGAACAAGC
TGGCCATCGTCCAGCATTAGTGTTAACGCCCACGATTTATAATCGTCAAACAGGGTTGTTAATTTGCTGCCCGCTCACAA
CAAAAGTAAAAGGCTATCCTTTCGAAGTCAATATTGCAGGTACCCCCCAAAATGTTGTGCTTTCCGATCAAATTAAAAGC
CTTGACTGGCGTATTCGTCACGCTAAATTTAAAGGTAAAATTAACCATCATCAGTTAAGCGAAGTAAAAGCTAAAATTGC
GACTTTGCTACAAATCAGTGAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8782.21 Da        Isoelectric Point: 5.0351

>AT48482 WP_005710601.1 NZ_CP009158:1228914-1229153 [Glaesserella parasuis]
MQVAVKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Download         Length: 240 bp

>AT48482 NZ_CP009158:1228914-1229153 [Glaesserella parasuis]
ATGCAAGTGGCTGTCAAAAAATGGGGAAATAGCGTAGGCATTCGCATTCCTGCTTTATTATCTAATTCATTGAAATTACA
GGCAGAGAGTTTAGTCGATATTCAAGAAGTTGATGGTCGCCTAATTATTGAGCCGATTAAACCTAAATATAATTTAGAGC
AACTTCTTTCAGGTATTACTGAAGATAATTTGCACAATGAAGTCGATTTTGGCAAGCCTGTTGGCAAGGAAATGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A143CFE3

Antitoxin

Source ID Structure
AlphaFold DB U4SAI4

References