Detailed information of TA system TA048474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 301869..302502
• Replicon: chromosome
• Accession: NZ_CP009157
• Organism: Burkholderia pseudomallei strain TSV202

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: X994_RS16245
• Protein ID: WP_043299621.1
• Coordinates: 302089..302502 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: X994_RS16240
• Protein ID: WP_043300036.1
• Coordinates: 301869..302102 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X994_RS16225	297352..299208	-	1857	WP_043299618.1	TraI domain-containing protein	-
X994_RS16230	299550..301124	+	1575	WP_043299619.1	MASE1 domain-containing protein	-
X994_RS42570	301192..301653	-	462	WP_155033771.1	hypothetical protein	-
X994_RS16240	301869..302102	+	234	WP_043300036.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
X994_RS16245	302089..302502	+	414	WP_043299621.1	PIN domain-containing protein	Toxin
X994_RS16250	302536..302904	+	369	WP_043300037.1	DUF3742 family protein	-
X994_RS16255	302914..304419	-	1506	WP_043299622.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
X994_RS16260	304433..304774	-	342	WP_043299623.1	hypothetical protein	-
X994_RS16265	304777..306192	-	1416	WP_043299624.1	integrating conjugative element protein	-
X994_RS16270	306201..307145	-	945	WP_043299625.1	TIGR03756 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	285791..387462	101671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15384.89 Da
• Isoelectric Point: 6.2245

>T48474 WP_043299621.1 NZ_CP009157:302089-302502 [Burkholderia pseudomallei]
MSGADKTKVFVDSNVVLYLLSGDTVKADRAEALLRRRPTISVQVLNEVTNVCVRKLGMAWREVDQFLELVRQFCVVVPLT
IEMHDLARRIAERYGLAFYDACIVAAASIEGCRALYTEDMHHGLIIEDSLALRNPFA

Nucleotide

• Length: 414 bp

>T48474 NZ_CP009157:302089-302502 [Burkholderia pseudomallei]
ATGAGCGGGGCTGACAAGACGAAAGTGTTTGTGGACAGCAACGTCGTGTTGTATTTGCTATCCGGTGATACCGTGAAGGC
GGATCGTGCGGAGGCTCTGTTGCGGCGGCGGCCGACGATCAGCGTCCAGGTGCTAAATGAAGTCACGAACGTCTGCGTGC
GTAAGCTCGGTATGGCGTGGCGCGAGGTCGATCAGTTCCTGGAATTGGTGCGGCAGTTTTGCGTGGTCGTGCCACTCACA
ATCGAAATGCATGATTTGGCGCGGCGTATCGCCGAACGCTATGGTCTGGCGTTCTACGATGCGTGCATCGTAGCGGCGGC
GTCGATCGAAGGATGCCGTGCACTCTACACGGAAGACATGCATCACGGATTGATCATCGAAGATAGCTTGGCGTTGCGTA
ACCCTTTCGCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8775.05 Da
• Isoelectric Point: 7.0164

>AT48474 WP_043300036.1 NZ_CP009157:301869-302102 [Burkholderia pseudomallei]
MQVAKWGNSLAVRLPASLVEALELREGDDIEIIIDEPRTFAIRKKPGTDELLARLRHFRGKLPADFKFSRDEANERG

Nucleotide

• Length: 234 bp

>AT48474 NZ_CP009157:301869-302102 [Burkholderia pseudomallei]
ATGCAGGTTGCCAAATGGGGCAATAGCCTCGCGGTTCGCCTTCCCGCAAGCCTCGTGGAGGCACTCGAGTTGCGGGAGGG
GGACGATATCGAAATCATCATCGACGAACCCCGTACCTTCGCCATTCGCAAGAAACCGGGGACGGATGAACTGCTGGCGA
GGCTGCGGCACTTCCGCGGCAAGCTGCCGGCCGATTTCAAGTTCAGCCGGGACGAGGCGAATGAGCGGGGCTGA