48471

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 634126..634663 Replicon chromosome
Accession NZ_CP009156
Organism Burkholderia pseudomallei strain TSV202

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag X994_RS04150 Protein ID WP_004549731.1
Coordinates 634394..634663 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag X994_RS04145 Protein ID WP_004525420.1
Coordinates 634126..634410 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
X994_RS04120 629261..630496 - 1236 WP_100086160.1 c-type cytochrome -
X994_RS04125 630655..632115 - 1461 WP_004538070.1 metallophosphoesterase -
X994_RS04130 632362..632478 + 117 Protein_519 IS3 family transposase -
X994_RS35135 632705..633133 - 429 Protein_520 IS110 family transposase -
X994_RS04135 633096..633479 - 384 Protein_521 DDE-type integrase/transposase/recombinase -
X994_RS04145 634126..634410 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
X994_RS04150 634394..634663 - 270 WP_004549731.1 BrnT family toxin Toxin
X994_RS04155 635215..635490 + 276 WP_038726742.1 hypothetical protein -
X994_RS04160 635500..635631 + 132 WP_009923452.1 bacteriophage protein Gp48 -
X994_RS04170 635767..635988 + 222 WP_029248167.1 hypothetical protein -
X994_RS43155 635955..636254 + 300 WP_161788725.1 hypothetical protein -
X994_RS04180 636485..637067 - 583 Protein_528 hypothetical protein -
X994_RS04185 637323..637472 + 150 WP_004542626.1 bacteriophage protein Gp44 -
X994_RS43885 637469..638242 + 774 Protein_530 prohibitin family protein -
X994_RS43890 638240..638668 + 429 Protein_531 DDE-type integrase/transposase/recombinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 633096..640979 7883


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T48471 WP_004549731.1 NZ_CP009156:c634663-634394 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T48471 NZ_CP009156:c634663-634394 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT48471 WP_004525420.1 NZ_CP009156:c634410-634126 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT48471 NZ_CP009156:c634410-634126 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATTGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References