Detailed information of TA system TA048458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 964010..964695
• Replicon: plasmid pSymA
• Accession: NZ_CP009145
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DU99_RS22095
• Protein ID: WP_017263896.1
• Coordinates: 964276..964695 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: DU99_RS22090
• Protein ID: WP_013845417.1
• Coordinates: 964010..964279 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS22070	959646..960515	-	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-
DU99_RS22075	960632..961555	+	924	WP_014528622.1	LysR family transcriptional regulator	-
DU99_RS22085	962535..963410	+	876	WP_013845418.1	LysR family transcriptional regulator	-
DU99_RS22090	964010..964279	+	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DU99_RS22095	964276..964695	+	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
DU99_RS22100	964849..965262	-	414	WP_014528620.1	hypothetical protein	-
DU99_RS22105	965537..965848	+	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
DU99_RS22110	965866..966246	+	381	WP_003526626.1	transcriptional regulator	-
DU99_RS22115	966578..967264	+	687	Protein_939	ISNCY family transposase	-
DU99_RS22120	967288..967935	-	648	WP_017268072.1	autoinducer binding domain-containing protein	-
DU99_RS36205	968189..969146	-	958	Protein_941	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1466845	1466845
	-	flank	IS/Tn	-	-	966611..967390	779

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T48458 WP_017263896.1 NZ_CP009145:964276-964695 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T48458 NZ_CP009145:964276-964695 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT48458 WP_013845417.1 NZ_CP009145:964010-964279 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT48458 NZ_CP009145:964010-964279 [Sinorhizobium meliloti]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5