Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 462722..463329 Replicon plasmid pSymA
Accession NZ_CP009145
Organism Sinorhizobium meliloti strain RMO17

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag DU99_RS19670 Protein ID WP_017264738.1
Coordinates 463075..463329 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DU99_RS19665 Protein ID WP_040120685.1
Coordinates 462722..463078 (-) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DU99_RS19645 458230..459723 + 1494 WP_010968049.1 sugar ABC transporter ATP-binding protein -
DU99_RS19650 459740..460711 + 972 WP_040120683.1 ABC transporter permease -
DU99_RS19655 460714..461664 + 951 WP_040120684.1 ABC transporter permease -
DU99_RS19660 461694..462350 + 657 WP_010968046.1 SGNH/GDSL hydrolase family protein -
DU99_RS19665 462722..463078 - 357 WP_040120685.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DU99_RS19670 463075..463329 - 255 WP_017264738.1 type II toxin-antitoxin system HicA family toxin Toxin
DU99_RS19675 463671..464558 - 888 WP_040120686.1 LysR family transcriptional regulator -
DU99_RS19680 464647..465687 + 1041 WP_040120687.1 NAD(P)H-binding protein -
DU99_RS36185 465811..465969 + 159 WP_017276730.1 hypothetical protein -
DU99_RS35650 466055..466210 + 156 WP_153320124.1 hypothetical protein -
DU99_RS19685 466261..467133 - 873 WP_010968043.1 LysR family transcriptional regulator -
DU99_RS19690 467224..467928 + 705 WP_014527995.1 haloacid dehalogenase type II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..1466845 1466845


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9294.62 Da        Isoelectric Point: 9.8124

>T48456 WP_017264738.1 NZ_CP009145:c463329-463075 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Download         Length: 255 bp

>T48456 NZ_CP009145:c463329-463075 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13178.82 Da        Isoelectric Point: 5.2288

>AT48456 WP_040120685.1 NZ_CP009145:c463078-462722 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAAGHFAEARLSA

Download         Length: 357 bp

>AT48456 NZ_CP009145:c463078-462722 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGGCCATTTTGCCGAGGCGCGCCTGTCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References