Detailed information of TA system TA048455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 248780..249378
• Replicon: plasmid pSymA
• Accession: NZ_CP009145
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: DU99_RS18550
• Protein ID: WP_010967243.1
• Coordinates: 249085..249378 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: DU99_RS18545
• Protein ID: WP_010967242.1
• Coordinates: 248780..249088 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS18515	243956..244894	+	939	WP_014532024.1	nucleoside hydrolase	-
DU99_RS18520	244891..246417	+	1527	WP_040120589.1	amidohydrolase	-
DU99_RS18525	246608..246931	-	324	WP_003526806.1	DNA-binding transcriptional regulator	-
DU99_RS18530	246921..247280	-	360	WP_040120590.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DU99_RS35000	247650..248585	+	936	Protein_238	ParB N-terminal domain-containing protein	-
DU99_RS18545	248780..249088	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DU99_RS18550	249085..249378	+	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
DU99_RS18555	249611..250810	-	1200	WP_040120892.1	NAD-dependent formate dehydrogenase	-
DU99_RS18560	251186..251976	+	791	Protein_242	ParB N-terminal domain-containing protein	-
DU99_RS18565	252354..253130	-	777	WP_014528764.1	histidinol-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1466845	1466845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T48455 WP_010967243.1 NZ_CP009145:249085-249378 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T48455 NZ_CP009145:249085-249378 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT48455 WP_010967242.1 NZ_CP009145:248780-249088 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT48455 NZ_CP009145:248780-249088 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: Q930F0

Antitoxin

• AlphaFold DB: Q930F1