Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 167464..168044 Replicon plasmid pSymA
Accession NZ_CP009145
Organism Sinorhizobium meliloti strain RMO17

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag DU99_RS18115 Protein ID WP_010968151.1
Coordinates 167464..167847 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag DU99_RS18120 Protein ID WP_013851145.1
Coordinates 167844..168044 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DU99_RS18095 162708..163643 - 936 WP_014989617.1 LysR family transcriptional regulator -
DU99_RS18105 164640..166870 + 2231 Protein_149 S9 family peptidase -
DU99_RS18110 166908..167192 + 285 WP_100208865.1 hypothetical protein -
DU99_RS18115 167464..167847 - 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
DU99_RS18120 167844..168044 - 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
DU99_RS32905 168335..168736 + 402 WP_014531161.1 GFA family protein -
DU99_RS18125 168813..169649 - 837 WP_014531162.1 helix-turn-helix transcriptional regulator -
DU99_RS32910 169745..170068 + 324 WP_017266777.1 DUF1127 domain-containing protein -
DU99_RS18135 170107..170835 + 729 WP_014531163.1 class I SAM-dependent methyltransferase -
DU99_RS18140 170929..171075 + 147 WP_017269032.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..1466845 1466845


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T48453 WP_010968151.1 NZ_CP009145:c167847-167464 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T48453 NZ_CP009145:c167847-167464 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT48453 WP_013851145.1 NZ_CP009145:c168044-167844 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT48453 NZ_CP009145:c168044-167844 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References