Detailed information of TA system TA048450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2927341..2928107
• Replicon: chromosome
• Accession: NZ_CP009144
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: DU99_RS14110
• Protein ID: WP_003533488.1
• Coordinates: 2927625..2928107 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: DU99_RS14105
• Protein ID: WP_003533490.1
• Coordinates: 2927341..2927625 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS14090	2922811..2924199	+	1389	WP_003533497.1	lipopolysaccharide biosynthesis protein	-
DU99_RS14095	2924203..2925474	+	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
DU99_RS14100	2925490..2927136	-	1647	WP_010970088.1	AMP-binding protein	-
DU99_RS14105	2927341..2927625	+	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
DU99_RS14110	2927625..2928107	+	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
DU99_RS14120	2928572..2929081	+	510	WP_013844819.1	disulfide bond formation protein B	-
DU99_RS14125	2929113..2929670	-	558	WP_003533486.1	HNH endonuclease	-
DU99_RS14130	2929776..2930423	-	648	WP_010970091.1	DNA-3-methyladenine glycosylase	-
DU99_RS14135	2930576..2931472	+	897	WP_010970092.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
DU99_RS14140	2931491..2932366	-	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T48450 WP_003533488.1 NZ_CP009144:2927625-2928107 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T48450 NZ_CP009144:2927625-2928107 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT48450 WP_003533490.1 NZ_CP009144:2927341-2927625 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT48450 NZ_CP009144:2927341-2927625 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGTATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4