Detailed information of TA system TA048449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2696317..2696990
• Replicon: chromosome
• Accession: NZ_CP009144
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DU99_RS13000
• Protein ID: WP_010969926.1
• Coordinates: 2696586..2696990 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0GA10
• Locus tag: DU99_RS12995
• Protein ID: WP_003536345.1
• Coordinates: 2696317..2696589 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS12975	2692308..2693948	-	1641	WP_038439219.1	ABC transporter substrate-binding protein	-
DU99_RS12980	2693996..2695051	-	1056	WP_014529838.1	dipeptidase	-
DU99_RS12985	2695231..2695572	-	342	WP_003536339.1	EamA family transporter	-
DU99_RS12990	2695620..2696180	-	561	WP_010969925.1	TetR/AcrR family transcriptional regulator	-
DU99_RS12995	2696317..2696589	+	273	WP_003536345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DU99_RS13000	2696586..2696990	+	405	WP_010969926.1	type II toxin-antitoxin system VapC family toxin	Toxin
DU99_RS13005	2697118..2697510	+	393	WP_038439220.1	globin	-
DU99_RS13010	2697516..2697890	+	375	WP_010969927.1	DUF423 domain-containing protein	-
DU99_RS13015	2698013..2698225	+	213	WP_051968698.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DU99_RS13020	2698378..2699907	+	1530	WP_080599811.1	IS21 family transposase	-
DU99_RS13025	2699920..2700657	+	738	WP_012477343.1	IS21-like element helper ATPase IstB	-
DU99_RS13030	2700993..2701307	-	315	WP_003536354.1	antibiotic biosynthesis monooxygenase	-
DU99_RS13035	2701320..2701586	-	267	WP_038439222.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14747.98 Da
• Isoelectric Point: 5.1974

>T48449 WP_010969926.1 NZ_CP009144:2696586-2696990 [Sinorhizobium meliloti]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T48449 NZ_CP009144:2696586-2696990 [Sinorhizobium meliloti]
TTGAACGGCTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10334.02 Da
• Isoelectric Point: 6.3950

>AT48449 WP_003536345.1 NZ_CP009144:2696317-2696589 [Sinorhizobium meliloti]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT48449 NZ_CP009144:2696317-2696589 [Sinorhizobium meliloti]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Structures

Antitoxin

• AlphaFold DB: H0GA10