Detailed information of TA system TA048448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2232325..2232979
• Replicon: chromosome
• Accession: NZ_CP009144
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: DU99_RS10690
• Protein ID: WP_003530010.1
• Coordinates: 2232325..2232513 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: DU99_RS10695
• Protein ID: WP_010969659.1
• Coordinates: 2232518..2232979 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS10675	2228891..2230201	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
DU99_RS10680	2230226..2231197	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
DU99_RS10685	2231378..2232148	-	771	WP_010969658.1	hypothetical protein	-
DU99_RS10690	2232325..2232513	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
DU99_RS10695	2232518..2232979	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DU99_RS10700	2233385..2233843	+	459	WP_038439089.1	SMI1/KNR4 family protein	-
DU99_RS32510	2233878..2234048	-	171	WP_144245252.1	hypothetical protein	-
DU99_RS10705	2234127..2235182	+	1056	WP_017275254.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
DU99_RS10710	2235409..2235833	-	425	Protein_2096	ATP-dependent DNA ligase	-
DU99_RS36110	2235926..2236096	+	171	WP_015007719.1	hypothetical protein	-
DU99_RS10715	2236089..2236457	+	369	WP_038439091.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T48448 WP_003530010.1 NZ_CP009144:2232325-2232513 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T48448 NZ_CP009144:2232325-2232513 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT48448 WP_010969659.1 NZ_CP009144:2232518-2232979 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT48448 NZ_CP009144:2232518-2232979 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0