Detailed information of TA system TA048447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 1385493..1386067
• Replicon: chromosome
• Accession: NZ_CP009144
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92QN4
• Locus tag: DU99_RS06480
• Protein ID: WP_010969154.1
• Coordinates: 1385493..1385858 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92QN3
• Locus tag: DU99_RS06485
• Protein ID: WP_010969155.1
• Coordinates: 1385852..1386067 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS06455	1380633..1382075	+	1443	WP_010969150.1	NADH-quinone oxidoreductase subunit NuoN	-
DU99_RS06460	1382072..1382839	+	768	WP_003531861.1	biotin--[acetyl-CoA-carboxylase] ligase	-
DU99_RS06465	1382867..1384534	+	1668	WP_010969151.1	ribonuclease J	-
DU99_RS06470	1384593..1384997	+	405	WP_010969152.1	methylmalonyl-CoA epimerase	-
DU99_RS06475	1385137..1385406	+	270	WP_014526683.1	DUF1467 family protein	-
DU99_RS06480	1385493..1385858	-	366	WP_010969154.1	type II toxin-antitoxin system VapC family toxin	Toxin
DU99_RS06485	1385852..1386067	-	216	WP_010969155.1	ribbon-helix-helix protein, CopG family	Antitoxin
DU99_RS06490	1386135..1386362	-	228	WP_017264047.1	hypothetical protein	-
DU99_RS06495	1386778..1388106	+	1329	WP_003531873.1	proline--tRNA ligase	-
DU99_RS06500	1388127..1389437	+	1311	WP_014529722.1	lipoprotein-releasing ABC transporter permease subunit	-
DU99_RS06505	1389455..1390141	+	687	WP_010969157.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13592.57 Da
• Isoelectric Point: 5.1787

>T48447 WP_010969154.1 NZ_CP009144:c1385858-1385493 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Nucleotide

• Length: 366 bp

>T48447 NZ_CP009144:c1385858-1385493 [Sinorhizobium meliloti]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8381.51 Da
• Isoelectric Point: 8.6052

>AT48447 WP_010969155.1 NZ_CP009144:c1386067-1385852 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Nucleotide

• Length: 216 bp

>AT48447 NZ_CP009144:c1386067-1385852 [Sinorhizobium meliloti]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTTCGCGGCGAATGGTAG

Structures

Toxin

• AlphaFold DB: Q92QN4

Antitoxin

• AlphaFold DB: Q92QN3