48440

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1085756..1086293 Replicon chromosome
Accession NZ_CP009127
Organism Burkholderia pseudomallei strain BSR

Toxin (Protein)


Gene name brnT Uniprot ID A0A0F6L9W9
Locus tag DU27_RS04540 Protein ID WP_009971435.1
Coordinates 1085756..1086025 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag DU27_RS04545 Protein ID WP_004525420.1
Coordinates 1086009..1086293 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DU27_RS41005 1081752..1082180 - 429 Protein_893 DDE-type integrase/transposase/recombinase -
DU27_RS41010 1082178..1082951 - 774 Protein_894 prohibitin family protein -
DU27_RS04505 1082948..1083085 - 138 WP_009971437.1 hypothetical protein -
DU27_RS04510 1083353..1083934 + 582 WP_004542548.1 hypothetical protein -
DU27_RS40380 1084165..1084383 - 219 WP_004552392.1 hypothetical protein -
DU27_RS04520 1084431..1084652 - 222 WP_004538072.1 hypothetical protein -
DU27_RS04535 1084929..1085204 - 276 WP_004537565.1 bacteriophage protein Gp49 -
DU27_RS04540 1085756..1086025 + 270 WP_009971435.1 BrnT family toxin Toxin
DU27_RS04545 1086009..1086293 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
DU27_RS04555 1086940..1087323 + 384 Protein_902 DDE-type integrase/transposase/recombinase -
DU27_RS32680 1087286..1087714 + 429 Protein_903 IS110 family transposase -
DU27_RS04560 1087941..1088057 - 117 Protein_904 IS3 family transposase -
DU27_RS04565 1088304..1089764 + 1461 WP_004538070.1 metallophosphoesterase -
DU27_RS04570 1089923..1091185 + 1263 WP_004537187.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1079441..1093413 13972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10143.43 Da        Isoelectric Point: 5.6831

>T48440 WP_009971435.1 NZ_CP009127:1085756-1086025 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVVGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T48440 NZ_CP009127:1085756-1086025 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGGTCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT48440 WP_004525420.1 NZ_CP009127:1086009-1086293 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT48440 NZ_CP009127:1086009-1086293 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6L9W9

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References