Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2038..2557 Replicon plasmid pPA21A
Accession NZ_CP009126
Organism Pectobacterium atrosepticum strain 21A

Toxin (Protein)


Gene name parE Uniprot ID A0A1X0W371
Locus tag GZ59_RS22225 Protein ID WP_040118335.1
Coordinates 2038..2322 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1X0W393
Locus tag GZ59_RS22230 Protein ID WP_008815935.1
Coordinates 2315..2557 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ59_RS22215 659..904 - 246 WP_040118333.1 hypothetical protein -
GZ59_RS22220 987..1640 - 654 WP_040118334.1 ATPase -
GZ59_RS22225 2038..2322 - 285 WP_040118335.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GZ59_RS22230 2315..2557 - 243 WP_008815935.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
GZ59_RS22235 3130..4047 - 918 WP_050880439.1 replication initiator protein RepA -
GZ59_RS22240 4555..4917 - 363 WP_040118336.1 helix-turn-helix domain-containing protein -
GZ59_RS22245 5188..5643 + 456 WP_040118337.1 transcriptional antiterminator NusG -
GZ59_RS22250 5723..5947 + 225 WP_040118338.1 hypothetical protein -
GZ59_RS22255 5968..6636 + 669 WP_040118339.1 lytic transglycosylase domain-containing protein -
GZ59_RS22260 6646..6939 + 294 WP_040118340.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32444 32444


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-88)

Antitoxin

Antitoxin ParD

(1-64)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10975.50 Da        Isoelectric Point: 6.2103

>T48439 WP_040118335.1 NZ_CP009126:c2322-2038 [Pectobacterium atrosepticum]
VYKLTERAADDFAGIYDYTLLKFGEAQADHYTDALEAFFETLAGMPDMGRDYHAVPGVMRIEFQRHAIFYTVRDTDILIA
RILHQQMNHKRHLL

Download         Length: 285 bp

>T48439 NZ_CP009126:c2322-2038 [Pectobacterium atrosepticum]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACCGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGATTACCATG
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTTTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8860.98 Da        Isoelectric Point: 7.0130

>AT48439 WP_008815935.1 NZ_CP009126:c2557-2315 [Pectobacterium atrosepticum]
MARTTSVTIGEQLDSFISRLIDSGRYGSASEVMRSALRLLEQQETNDEVVRQAVIAGLESGESSLSLRDIAAQRKLRHRV

Download         Length: 243 bp

>AT48439 NZ_CP009126:c2557-2315 [Pectobacterium atrosepticum]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATCAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGCTCTGCTCTTCGATTGCTGGAGCAACAGGAAACAAATGATGAAGTTGTTCGCCAGGCTG
TCATTGCCGGGCTGGAAAGTGGTGAAAGCTCACTATCACTGCGGGATATTGCAGCACAAAGAAAGCTGAGACATCGTGTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X0W371

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0W393

References