Detailed information of TA system TA048432

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 714128..714788
• Replicon: chromosome
• Accession: NZ_CP009125
• Organism: Pectobacterium atrosepticum strain 21A

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: Q6D959
• Locus tag: GZ59_RS03220
• Protein ID: WP_011092367.1
• Coordinates: 714375..714788 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: Q6D960
• Locus tag: GZ59_RS03215
• Protein ID: WP_011092366.1
• Coordinates: 714128..714394 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ59_RS03195	709935..710939	-	1005	WP_011092362.1	LacI family transcriptional regulator	-
GZ59_RS03200	710993..711601	-	609	WP_011092363.1	HD domain-containing protein	-
GZ59_RS03205	712020..712667	+	648	WP_011092364.1	hemolysin III family protein	-
GZ59_RS03210	712891..713892	-	1002	WP_039290019.1	tRNA-modifying protein YgfZ	-
GZ59_RS03215	714128..714394	+	267	WP_011092366.1	FAD assembly factor SdhE	Antitoxin
GZ59_RS03220	714375..714788	+	414	WP_011092367.1	protein YgfX	Toxin
GZ59_RS03225	715096..716031	+	936	WP_011092368.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
GZ59_RS03230	716149..716472	-	324	WP_011092369.1	hypothetical protein	-
GZ59_RS03235	716501..717019	-	519	WP_011092370.1	flavodoxin FldB	-
GZ59_RS23640	717286..717423	+	138	WP_155242714.1	hypothetical protein	-
GZ59_RS03240	717438..717884	-	447	WP_011092372.1	DUF1311 domain-containing protein	-
GZ59_RS22390	718085..718693	-	609	WP_011092373.1	hypothetical protein	-
GZ59_RS03250	718960..719460	-	501	WP_011092374.1	DUF4189 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16397.31 Da
• Isoelectric Point: 10.3509

>T48432 WP_011092367.1 NZ_CP009125:714375-714788 [Pectobacterium atrosepticum]
VAQWQCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGVLLALQSINDKERQQLWLASDSMGDDEWRHLRQLLLQQKHWAR

Nucleotide

• Length: 414 bp

>T48432 NZ_CP009125:714375-714788 [Pectobacterium atrosepticum]
GTGGCCCAGTGGCAATGTGACCTTCGTGTTTCCTGGCGCATGCAACTGCTGTCATTGGTTGTGCATGGCCTACTCGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGTTACGCATGGCTATGGCTATGCTTAGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAAAGGAATATCAAATCCCGACAGGGGGAAATCGTTTTGCTTAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTCAAACGACCGTGGCTTTTGAAAAATGGCGTGTTGTTGGCATTGCAATCTATCAACGATAAAGAAAG
ACAACAGCTGTGGCTGGCATCGGACAGTATGGGCGATGATGAGTGGCGCCATCTGCGTCAGCTTCTTTTGCAGCAGAAAC
ACTGGGCGCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10462.94 Da
• Isoelectric Point: 6.2223

>AT48432 WP_011092366.1 NZ_CP009125:714128-714394 [Pectobacterium atrosepticum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDSDKRAFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT48432 NZ_CP009125:714128-714394 [Pectobacterium atrosepticum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGTGAACTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACAGTGATAAGCGCGCGTTTGTACGCCTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGAAGGACGGAGAGTTGAAACGTATGATTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: Q6D959

Antitoxin

• AlphaFold DB: Q6D960