48427

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 800921..801445 Replicon chromosome
Accession NZ_CP009120
Organism Staphylococcus pseudintermedius strain 5912

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag SPS5912_RS03550 Protein ID WP_014613379.1
Coordinates 801092..801445 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag SPS5912_RS03545 Protein ID WP_014613378.1
Coordinates 800921..801091 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SPS5912_RS03520 796810..797283 + 474 WP_014613373.1 PH domain-containing protein -
SPS5912_RS03525 797276..798805 + 1530 WP_014613374.1 PH domain-containing protein -
SPS5912_RS03530 798789..799307 + 519 WP_014613375.1 PH domain-containing protein -
SPS5912_RS03535 799304..799654 + 351 WP_110178649.1 holo-ACP synthase -
SPS5912_RS03540 799688..800836 + 1149 WP_014613377.1 alanine racemase -
SPS5912_RS03545 800921..801091 + 171 WP_014613378.1 hypothetical protein Antitoxin
SPS5912_RS03550 801092..801445 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SPS5912_RS03555 801503..802507 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
SPS5912_RS03560 802586..802912 + 327 WP_014613381.1 anti-sigma factor antagonist -
SPS5912_RS03565 802912..803391 + 480 WP_031253870.1 anti-sigma B factor RsbW -
SPS5912_RS03570 803366..804136 + 771 WP_015729428.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T48427 WP_014613379.1 NZ_CP009120:801092-801445 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T48427 NZ_CP009120:801092-801445 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT48427 WP_014613378.1 NZ_CP009120:800921-801091 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT48427 NZ_CP009120:800921-801091 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References