Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4778799..4779418 Replicon chromosome
Accession NZ_CP009114
Organism Klebsiella pneumoniae strain carbapenem-resistant blaNDM-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FH42_RS23935 Protein ID WP_002892050.1
Coordinates 4778799..4779017 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FH42_RS23940 Protein ID WP_002892066.1
Coordinates 4779044..4779418 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH42_RS23900 4774846..4775109 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
FH42_RS23905 4775109..4775249 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FH42_RS23910 4775246..4775944 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
FH42_RS23915 4776045..4777496 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FH42_RS23920 4777471..4777941 - 471 WP_002892026.1 YlaC family protein -
FH42_RS30785 4777962..4778102 + 141 WP_004147370.1 hypothetical protein -
FH42_RS23930 4778074..4778640 - 567 WP_002892030.1 maltose O-acetyltransferase -
FH42_RS23935 4778799..4779017 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FH42_RS23940 4779044..4779418 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FH42_RS23945 4779904..4783050 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
FH42_RS23950 4783073..4784266 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T48423 WP_002892050.1 NZ_CP009114:c4779017-4778799 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T48423 NZ_CP009114:c4779017-4778799 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT48423 WP_002892066.1 NZ_CP009114:c4779418-4779044 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT48423 NZ_CP009114:c4779418-4779044 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References