Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4159557..4160367 Replicon chromosome
Accession NZ_CP009114
Organism Klebsiella pneumoniae strain carbapenem-resistant blaNDM-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag FH42_RS20870 Protein ID WP_002887280.1
Coordinates 4159834..4160367 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag FH42_RS20865 Protein ID WP_002887278.1
Coordinates 4159557..4159823 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH42_RS20840 4155432..4155776 - 345 WP_025987660.1 cation efflux system protein CusF -
FH42_RS20845 4155794..4157176 - 1383 WP_019724904.1 efflux transporter outer membrane subunit -
FH42_RS20855 4157348..4158031 + 684 WP_004214143.1 copper response regulator transcription factor CusR -
FH42_RS20860 4158021..4159454 + 1434 WP_019724905.1 Cu(+)/Ag(+) sensor histidine kinase -
FH42_RS20865 4159557..4159823 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
FH42_RS20870 4159834..4160367 + 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
FH42_RS20875 4160415..4161536 - 1122 WP_009309849.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T48422 WP_002887280.1 NZ_CP009114:4159834-4160367 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T48422 NZ_CP009114:4159834-4160367 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTTTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT48422 WP_002887278.1 NZ_CP009114:4159557-4159823 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT48422 NZ_CP009114:4159557-4159823 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References