Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4115749..4116440 Replicon chromosome
Accession NZ_CP009114
Organism Klebsiella pneumoniae strain carbapenem-resistant blaNDM-1

Toxin (Protein)


Gene name ykfI Uniprot ID A0A483YFJ1
Locus tag FH42_RS20635 Protein ID WP_025987721.1
Coordinates 4116099..4116440 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2A5MHA6
Locus tag FH42_RS20630 Protein ID WP_019725272.1
Coordinates 4115749..4116075 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH42_RS20605 4111276..4112364 + 1089 WP_019725277.1 hypothetical protein -
FH42_RS20610 4112447..4113337 + 891 WP_019725276.1 50S ribosome-binding GTPase -
FH42_RS20615 4113902..4114591 + 690 WP_009309812.1 hypothetical protein -
FH42_RS20620 4114803..4115249 + 447 WP_025987722.1 antirestriction protein -
FH42_RS20625 4115259..4115735 + 477 WP_019725273.1 DNA repair protein RadC -
FH42_RS20630 4115749..4116075 + 327 WP_019725272.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FH42_RS20635 4116099..4116440 + 342 WP_025987721.1 TA system toxin CbtA family protein Toxin
FH42_RS20640 4116555..4117388 + 834 WP_025987720.1 DUF4942 domain-containing protein -
FH42_RS20645 4117475..4117786 + 312 WP_073568733.1 hypothetical protein -
FH42_RS20650 4118260..4119495 + 1236 WP_124044824.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4094730..4134045 39315


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(8-107)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12867.90 Da        Isoelectric Point: 8.5195

>T48421 WP_025987721.1 NZ_CP009114:4116099-4116440 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Download         Length: 342 bp

>T48421 NZ_CP009114:4116099-4116440 [Klebsiella pneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGGCAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12276.01 Da        Isoelectric Point: 6.9560

>AT48421 WP_019725272.1 NZ_CP009114:4115749-4116075 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Download         Length: 327 bp

>AT48421 NZ_CP009114:4115749-4116075 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGGTGACGCCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YFJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MHA6

References