Detailed information of TA system TA048404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 408845..409815
• Replicon: plasmid pR1CP1
• Accession: NZ_CP009112
• Organism: Rhodococcus opacus strain 1CP

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A1B1KHR8
• Locus tag: R1CP_RS36600
• Protein ID: WP_065493618.1
• Coordinates: 408845..409435 (-)
• Length: 197 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: K8X4T1
• Locus tag: R1CP_RS36605
• Protein ID: WP_005265240.1
• Coordinates: 409432..409815 (-)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R1CP_RS36590	405800..407506	-	1707	WP_065493617.1	DEAD/DEAH box helicase	-
R1CP_RS36595	408094..408309	+	216	WP_065493871.1	hypothetical protein	-
R1CP_RS40465	408322..408516	+	195	WP_081315648.1	DUF4193 family protein	-
R1CP_RS36600	408845..409435	-	591	WP_065493618.1	zeta toxin family protein	Toxin
R1CP_RS36605	409432..409815	-	384	WP_005265240.1	hypothetical protein	Antitoxin
R1CP_RS36610	410476..411479	-	1004	Protein_350	acyl-CoA/acyl-ACP dehydrogenase	-
R1CP_RS36615	411737..413047	-	1311	WP_065493619.1	Rep protein	-
R1CP_RS36620	413422..413739	-	318	WP_065493620.1	hypothetical protein	-
R1CP_RS36625	413736..414230	-	495	WP_061699536.1	hypothetical protein	-
R1CP_RS36630	414227..414535	-	309	WP_065493621.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..885383	885383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-142)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-106)

Sequences

Toxin

Protein

• Length: 197 a.a.
• Molecular weight: 21536.50 Da
• Isoelectric Point: 8.0266

>T48404 WP_065493618.1 NZ_CP009112:c409435-408845 [Rhodococcus opacus]
VKRLDLVAGCNGAGKSTFVAFTLAPLLPASPLVNADEIATQRWPADPAIHSHEAAEIAAKTRAHLLTLGKSFIAATVFSH
PSKLELIDEARTHDYTVVLHVVMIPEELAVQRVRYRVRAGGHEVPEDKIRQRYQRIWGLLAQAFPRCDHATVYDNSSSTG
PRIVAQFTDGHLVGEATWPAWTPAKLTSHTSRGEHP

Nucleotide

• Length: 591 bp

>T48404 NZ_CP009112:c409435-408845 [Rhodococcus opacus]
GTGAAACGACTCGACCTGGTGGCCGGCTGCAACGGCGCCGGCAAGTCCACCTTCGTGGCCTTCACCCTCGCCCCCCTGCT
GCCAGCCAGCCCGCTCGTCAATGCCGACGAAATCGCCACACAACGATGGCCGGCAGATCCGGCCATCCACTCCCATGAGG
CGGCGGAGATCGCCGCGAAGACCCGAGCACACCTGCTGACCCTGGGGAAATCGTTCATCGCCGCGACGGTGTTCTCCCAT
CCGTCGAAACTGGAGCTGATCGACGAAGCGCGCACACACGACTACACCGTCGTCTTACATGTGGTGATGATTCCCGAGGA
ACTAGCGGTCCAGCGTGTGCGCTACCGGGTCCGAGCCGGCGGACACGAAGTGCCCGAAGACAAGATCCGCCAGCGATACC
AAAGAATATGGGGTCTGCTCGCTCAGGCGTTCCCACGGTGTGATCATGCGACCGTGTACGACAACAGCTCGAGCACCGGC
CCGCGCATCGTCGCCCAGTTCACCGACGGACACCTCGTCGGCGAAGCCACCTGGCCCGCCTGGACACCCGCAAAACTGAC
CTCGCACACCAGCCGGGGCGAGCACCCATAG

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 13694.21 Da
• Isoelectric Point: 4.9627

>AT48404 WP_005265240.1 NZ_CP009112:c409815-409432 [Rhodococcus opacus]
MHSTGDKVTRLAADLVASAMAEGERQHRTGRQQLEHWARVGRAVSSRQTAARRRVEDALAGRLPLGELGPEEGTVFNAEI
NAAIEEQLATTDYGATLAAQGITTVALSEDGQIIEYRPDGTRTILPE

Nucleotide

• Length: 384 bp

>AT48404 NZ_CP009112:c409815-409432 [Rhodococcus opacus]
ATGCACAGCACTGGCGACAAGGTCACCCGCCTGGCAGCCGACCTGGTGGCCAGCGCCATGGCCGAGGGAGAACGGCAACA
CCGCACCGGACGCCAACAACTCGAACACTGGGCCCGGGTGGGACGTGCGGTCTCGAGCCGACAGACCGCGGCCCGCCGCC
GAGTAGAAGACGCCCTCGCCGGCCGGTTGCCGCTCGGCGAACTCGGACCCGAAGAAGGCACAGTGTTCAACGCCGAGATC
AACGCGGCGATCGAGGAACAACTGGCCACCACCGACTACGGCGCTACGCTCGCCGCCCAGGGAATCACCACCGTCGCGCT
GTCCGAGGACGGTCAGATCATCGAGTACCGTCCCGACGGCACGCGCACAATCCTGCCCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A1B1KHR8

Antitoxin

• AlphaFold DB: K8X4T1