48403

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1498632..1499268 Replicon chromosome
Accession NZ_CP009108
Organism Bacillus altitudinis strain GR-8

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag ID12_RS07450 Protein ID WP_003214169.1
Coordinates 1498918..1499268 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag ID12_RS07445 Protein ID WP_012008995.1
Coordinates 1498632..1498913 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID12_RS07425 1494786..1495391 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
ID12_RS07430 1495485..1495850 + 366 WP_017366663.1 holo-ACP synthase -
ID12_RS07435 1496011..1497027 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
ID12_RS07440 1497156..1498334 + 1179 WP_050826986.1 alanine racemase -
ID12_RS07445 1498632..1498913 + 282 WP_012008995.1 hypothetical protein Antitoxin
ID12_RS07450 1498918..1499268 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ID12_RS07455 1499383..1500213 + 831 WP_007496484.1 STAS domain-containing protein -
ID12_RS07460 1500218..1500586 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
ID12_RS07465 1500589..1500990 + 402 WP_019744214.1 anti-sigma regulatory factor -
ID12_RS07470 1501001..1502008 + 1008 WP_008346904.1 PP2C family protein-serine/threonine phosphatase -
ID12_RS07475 1502068..1502397 + 330 WP_019744215.1 anti-sigma factor antagonist -
ID12_RS07480 1502394..1502882 + 489 WP_019744216.1 anti-sigma B factor RsbW -
ID12_RS07485 1502848..1503636 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
ID12_RS07490 1503636..1504235 + 600 WP_017358391.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T48403 WP_003214169.1 NZ_CP009108:1498918-1499268 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T48403 NZ_CP009108:1498918-1499268 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAAATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT48403 WP_012008995.1 NZ_CP009108:1498632-1498913 [Bacillus altitudinis]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT48403 NZ_CP009108:1498632-1498913 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTGATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References