Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 80440..80965 Replicon plasmid pO104_H21
Accession NZ_CP009107
Organism Escherichia coli strain 94-3024

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0F6YRT4
Locus tag HW43_RS24600 Protein ID WP_021293254.1
Coordinates 80440..80745 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag HW43_RS24605 Protein ID WP_000813639.1
Coordinates 80747..80965 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW43_RS26690 76434..76607 + 174 Protein_84 DUF4113 domain-containing protein -
HW43_RS24575 76609..77025 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
HW43_RS24580 77018..77998 - 981 WP_000688510.1 plasmid segregation protein ParM -
HW43_RS24585 78413..78721 - 309 WP_000030199.1 hypothetical protein -
HW43_RS24590 78808..79452 - 645 WP_001144036.1 ParA family protein -
HW43_RS24595 79633..80439 - 807 WP_000016966.1 site-specific integrase -
HW43_RS24600 80440..80745 - 306 WP_021293254.1 type II toxin-antitoxin system toxin CcdB Toxin
HW43_RS24605 80747..80965 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HW43_RS24615 81561..81821 + 261 WP_000343102.1 hypothetical protein -
HW43_RS24620 81818..82408 + 591 WP_021293255.1 hypothetical protein -
HW43_RS24625 82426..82773 - 348 WP_033817147.1 hypothetical protein -
HW43_RS24630 82902..83237 + 336 WP_021292998.1 colicin transporter -
HW43_RS24635 83244..83614 - 371 Protein_96 colicin -
HW43_RS24640 83674..84405 - 732 WP_000850413.1 complement resistance protein TraT -
HW43_RS28150 84682..84792 - 111 Protein_98 IS481 family transposase -
HW43_RS26700 84796..85010 - 215 Protein_99 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..161447 161447


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11634.45 Da        Isoelectric Point: 7.2010

>T48402 WP_021293254.1 NZ_CP009107:c80745-80440 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSGIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T48402 NZ_CP009107:c80745-80440 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGGCATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT48402 WP_000813639.1 NZ_CP009107:c80965-80747 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT48402 NZ_CP009107:c80965-80747 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6YRT4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References