Detailed information of TA system TA048400

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4105585..4106383
• Replicon: chromosome
• Accession: NZ_CP009106
• Organism: Escherichia coli strain 94-3024

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HW43_RS20295
• Protein ID: WP_000854902.1
• Coordinates: 4106006..4106383 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: HW43_RS20290
• Protein ID: WP_001280952.1
• Coordinates: 4105585..4105959 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW43_RS20255	4102421..4102876	+	456	WP_000581507.1	hypothetical protein	-
HW43_RS20260	4102955..4103188	+	234	WP_001119729.1	DUF905 family protein	-
HW43_RS20265	4103288..4104106	+	819	WP_001234626.1	DUF945 domain-containing protein	-
HW43_RS20270	4104161..4104646	+	486	WP_000849590.1	antirestriction protein	-
HW43_RS20275	4104662..4105138	+	477	WP_001186725.1	RadC family protein	-
HW43_RS20280	4105201..4105422	+	222	WP_000692312.1	DUF987 domain-containing protein	-
HW43_RS20290	4105585..4105959	+	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HW43_RS20295	4106006..4106383	+	378	WP_000854902.1	TA system toxin CbtA family protein	Toxin
HW43_RS20300	4106380..4106868	+	489	WP_000779175.1	hypothetical protein	-
HW43_RS20305	4106880..4107077	+	198	WP_000839249.1	DUF957 domain-containing protein	-
HW43_RS20310	4107162..4108004	+	843	WP_001280537.1	DUF4942 domain-containing protein	-
HW43_RS20315	4108347..4108883	-	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
HW43_RS20320	4108885..4110063	-	1179	WP_000094963.1	type II secretion system protein GspL	-
HW43_RS20325	4110060..4111037	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14307.29 Da
• Isoelectric Point: 7.2922

>T48400 WP_000854902.1 NZ_CP009106:4106006-4106383 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
NACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T48400 NZ_CP009106:4106006-4106383 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AATGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT48400 WP_001280952.1 NZ_CP009106:4105585-4105959 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT48400 NZ_CP009106:4105585-4105959 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A093DMQ4