Detailed information of TA system TA048399

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3948960..3949614
• Replicon: chromosome
• Accession: NZ_CP009106
• Organism: Escherichia coli strain 94-3024

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: HW43_RS19460
• Protein ID: WP_000244777.1
• Coordinates: 3948960..3949367 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HW43_RS19465
• Protein ID: WP_000354049.1
• Coordinates: 3949348..3949614 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW43_RS19440	3944917..3946650	-	1734	WP_000813215.1	single-stranded-DNA-specific exonuclease RecJ	-
HW43_RS19445	3946656..3947366	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HW43_RS19450	3947391..3948287	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
HW43_RS19455	3948399..3948920	+	522	WP_001055874.1	flavodoxin FldB	-
HW43_RS19460	3948960..3949367	-	408	WP_000244777.1	toxin CptA	Toxin
HW43_RS19465	3949348..3949614	-	267	WP_000354049.1	FAD assembly factor SdhE	Antitoxin
HW43_RS19470	3949857..3950837	+	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
HW43_RS19475	3950914..3951573	-	660	WP_000250274.1	hemolysin III family protein	-
HW43_RS19480	3951737..3952048	-	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
HW43_RS19485	3952093..3953526	+	1434	WP_001363803.1	6-phospho-beta-glucosidase BglA	-
HW43_RS19490	3953583..3954326	-	744	WP_000951951.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T48399 WP_000244777.1 NZ_CP009106:c3949367-3948960 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T48399 NZ_CP009106:c3949367-3948960 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10533.09 Da
• Isoelectric Point: 5.1599

>AT48399 WP_000354049.1 NZ_CP009106:c3949614-3949348 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFVRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT48399 NZ_CP009106:c3949614-3949348 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTGTTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7