Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3492946..3493571 Replicon chromosome
Accession NZ_CP009106
Organism Escherichia coli strain 94-3024

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HW43_RS17230 Protein ID WP_000911329.1
Coordinates 3492946..3493344 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HW43_RS17235 Protein ID WP_000450524.1
Coordinates 3493344..3493571 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW43_RS17210 3488853..3489053 + 201 WP_000383836.1 YpfN family protein -
HW43_RS17215 3489134..3489832 - 699 WP_000679812.1 esterase -
HW43_RS17220 3489906..3491921 - 2016 WP_000829335.1 tRNA cytosine(34) acetyltransferase TmcA -
HW43_RS17225 3491936..3492799 - 864 WP_001267507.1 neutral zinc metallopeptidase -
HW43_RS17230 3492946..3493344 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
HW43_RS17235 3493344..3493571 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HW43_RS17240 3493727..3494440 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HW43_RS17250 3494653..3495687 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HW43_RS17255 3495704..3496582 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HW43_RS17260 3496728..3497300 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HW43_RS17265 3497300..3497770 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T48397 WP_000911329.1 NZ_CP009106:c3493344-3492946 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T48397 NZ_CP009106:c3493344-3492946 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT48397 WP_000450524.1 NZ_CP009106:c3493571-3493344 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT48397 NZ_CP009106:c3493571-3493344 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References