Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 85423..85970 Replicon plasmid pO104_H7
Accession NZ_CP009105
Organism Escherichia coli strain RM9387

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag HW42_RS24600 Protein ID WP_000969996.1
Coordinates 85423..85704 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag HW42_RS24605 Protein ID WP_000079941.1
Coordinates 85701..85970 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW42_RS24580 80445..82559 - 2115 WP_123129447.1 peptidase domain-containing ABC transporter -
HW42_RS24585 82534..83808 - 1275 WP_024192804.1 HlyD family secretion protein -
HW42_RS28100 84219..84305 + 87 Protein_92 colicin-Ia domain protein -
HW42_RS24590 84454..84711 + 258 WP_040116958.1 type II toxin-antitoxin system antitoxin PemI -
HW42_RS24595 84680..85045 + 366 WP_148307810.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
HW42_RS24600 85423..85704 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HW42_RS24605 85701..85970 - 270 WP_000079941.1 hypothetical protein Antitoxin
HW42_RS24615 86880..87911 - 1032 WP_040116960.1 replication initiation protein -
HW42_RS24620 88613..89254 + 642 WP_077784641.1 transcription termination factor NusG -
HW42_RS24625 89395..90060 + 666 WP_001536608.1 hypothetical protein -
HW42_RS24630 90327..90947 + 621 WP_040116961.1 DUF1449 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..168318 168318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T48378 WP_000969996.1 NZ_CP009105:c85704-85423 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T48378 NZ_CP009105:c85704-85423 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT48378 WP_000079941.1 NZ_CP009105:c85970-85701 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT48378 NZ_CP009105:c85970-85701 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References