Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 62012..62537 Replicon plasmid pO104_H7
Accession NZ_CP009105
Organism Escherichia coli strain RM9387

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HW42_RS24465 Protein ID WP_001159871.1
Coordinates 62012..62317 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0F6YRL5
Locus tag HW42_RS24470 Protein ID WP_038355956.1
Coordinates 62319..62537 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW42_RS24440 58188..58604 - 417 WP_040116952.1 plasmid partitioning/stability family protein -
HW42_RS24445 58597..59577 - 981 WP_000688510.1 plasmid segregation protein ParM -
HW42_RS24450 59984..60292 - 309 WP_000030199.1 hypothetical protein -
HW42_RS24455 60379..61023 - 645 WP_001144036.1 ParA family protein -
HW42_RS24460 61205..62011 - 807 WP_040116953.1 site-specific integrase -
HW42_RS24465 62012..62317 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HW42_RS24470 62319..62537 - 219 WP_038355956.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HW42_RS24480 63132..63389 + 258 WP_000343085.1 hypothetical protein -
HW42_RS24485 63389..63979 + 591 WP_000194574.1 hypothetical protein -
HW42_RS26910 63999..64342 - 344 Protein_71 hypothetical protein -
HW42_RS24490 64461..64808 + 348 WP_000762580.1 hypothetical protein -
HW42_RS24495 64826..66706 - 1881 WP_001283354.1 colicin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..168318 168318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T48377 WP_001159871.1 NZ_CP009105:c62317-62012 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T48377 NZ_CP009105:c62317-62012 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTATCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8400.36 Da        Isoelectric Point: 4.7405

>AT48377 WP_038355956.1 NZ_CP009105:c62537-62319 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWRAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT48377 NZ_CP009105:c62537-62319 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCGGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6YRL5

References