Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 53819..54393 Replicon plasmid pO104_H7
Accession NZ_CP009105
Organism Escherichia coli strain RM9387

Toxin (Protein)


Gene name relE Uniprot ID A0A0F6SKD8
Locus tag HW42_RS24410 Protein ID WP_000421260.1
Coordinates 53819..54109 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag HW42_RS24415 Protein ID WP_001178089.1
Coordinates 54109..54393 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW42_RS28075 49471..49644 + 174 Protein_52 Tn3 family transposase -
HW42_RS24380 49841..50845 + 1005 Protein_53 IS110 family transposase -
HW42_RS28315 51345..51530 - 186 WP_169124752.1 hypothetical protein -
HW42_RS24395 52537..53743 - 1207 Protein_55 IS3 family transposase -
HW42_RS24410 53819..54109 - 291 WP_000421260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HW42_RS24415 54109..54393 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
HW42_RS24420 55017..55208 - 192 WP_001027503.1 hypothetical protein -
HW42_RS28325 55260..55454 - 195 WP_159454916.1 hypothetical protein -
HW42_RS24430 56536..56913 + 378 WP_040116951.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HW42_RS24435 56913..58186 + 1274 Protein_61 translesion error-prone DNA polymerase V subunit UmuC -
HW42_RS24440 58188..58604 - 417 WP_040116952.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..168318 168318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11356.12 Da        Isoelectric Point: 9.3504

>T48376 WP_000421260.1 NZ_CP009105:c54109-53819 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELYGQTIY
VLRLWHTRENRYEPAP

Download         Length: 291 bp

>T48376 NZ_CP009105:c54109-53819 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTTATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTATGAACCGGCCCCTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT48376 WP_001178089.1 NZ_CP009105:c54393-54109 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT48376 NZ_CP009105:c54393-54109 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTTGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6SKD8

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References