Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2373536..2374174 Replicon chromosome
Accession NZ_CP009104
Organism Escherichia coli strain RM9387

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HW42_RS11875 Protein ID WP_000813794.1
Coordinates 2373536..2373712 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HW42_RS11880 Protein ID WP_001270286.1
Coordinates 2373758..2374174 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW42_RS11855 2369155..2370369 - 1215 WP_071609973.1 BenE family transporter YdcO -
HW42_RS11860 2370422..2370958 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HW42_RS11865 2371031..2372992 + 1962 WP_001442195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HW42_RS11870 2373084..2373314 - 231 WP_000494244.1 YncJ family protein -
HW42_RS11875 2373536..2373712 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HW42_RS11880 2373758..2374174 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HW42_RS11885 2374253..2375659 + 1407 WP_000760663.1 PLP-dependent aminotransferase family protein -
HW42_RS11890 2375904..2377049 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HW42_RS11895 2377067..2378080 + 1014 WP_001542893.1 ABC transporter ATP-binding protein -
HW42_RS11900 2378081..2379022 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2367967..2369115 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T48361 WP_000813794.1 NZ_CP009104:2373536-2373712 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T48361 NZ_CP009104:2373536-2373712 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT48361 WP_001270286.1 NZ_CP009104:2373758-2374174 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT48361 NZ_CP009104:2373758-2374174 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References