Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 597472..598067 Replicon chromosome
Accession NZ_CP009104
Organism Escherichia coli strain RM9387

Toxin (Protein)


Gene name chpB Uniprot ID A0A3L3YDL1
Locus tag HW42_RS02865 Protein ID WP_000239576.1
Coordinates 597717..598067 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HW42_RS02860 Protein ID WP_001223208.1
Coordinates 597472..597723 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW42_RS02850 593137..596916 + 3780 WP_000060896.1 autotransporter assembly complex protein TamB -
HW42_RS02855 596919..597260 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HW42_RS02860 597472..597723 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HW42_RS02865 597717..598067 + 351 WP_000239576.1 endoribonuclease toxin ChpB Toxin
HW42_RS02870 598147..598677 - 531 WP_000055075.1 inorganic diphosphatase -
HW42_RS02875 598987..599943 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HW42_RS02880 600083..601585 + 1503 WP_000205795.1 sugar ABC transporter ATP-binding protein -
HW42_RS02885 601599..602621 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12566.53 Da        Isoelectric Point: 6.2232

>T48358 WP_000239576.1 NZ_CP009104:597717-598067 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPIRQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T48358 NZ_CP009104:597717-598067 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTAGGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT48358 WP_001223208.1 NZ_CP009104:597472-597723 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT48358 NZ_CP009104:597472-597723 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L3YDL1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References