Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3136648..3137252 Replicon chromosome
Accession NZ_CP009101
Organism Mycobacterium tuberculosis strain ZMC13-88

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JE53_RS14920 Protein ID WP_003414492.1
Coordinates 3136648..3137040 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JE53_RS14925 Protein ID WP_003414495.1
Coordinates 3137037..3137252 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE53_RS14890 3131798..3132586 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
JE53_RS14895 3132920..3133465 - 546 WP_014584866.1 DUF1802 family protein -
JE53_RS14900 3133737..3134621 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JE53_RS14905 3134624..3135511 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JE53_RS14910 3135816..3136361 - 546 WP_009939112.1 DUF1802 family protein -
JE53_RS14915 3136358..3136627 - 270 WP_003414489.1 DUF2277 family protein -
JE53_RS14920 3136648..3137040 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JE53_RS14925 3137037..3137252 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JE53_RS14930 3137299..3138048 + 750 WP_003902358.1 enoyl-CoA hydratase -
JE53_RS14935 3138127..3139209 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
JE53_RS14940 3139202..3140512 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
JE53_RS14945 3140515..3141342 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JE53_RS14950 3141339..3142250 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T48347 WP_003414492.1 NZ_CP009101:c3137040-3136648 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T48347 NZ_CP009101:c3137040-3136648 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT48347 WP_003414495.1 NZ_CP009101:c3137252-3137037 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT48347 NZ_CP009101:c3137252-3137037 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References