Detailed information of TA system TA048325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194671..2195374
• Replicon: chromosome
• Accession: NZ_CP009101
• Organism: Mycobacterium tuberculosis strain ZMC13-88

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JE53_RS10175
• Protein ID: WP_003409778.1
• Coordinates: 2194671..2195000 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JE53_RS21325
• Protein ID: WP_003409780.1
• Coordinates: 2194997..2195374 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE53_RS10155	2191054..2192124	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
JE53_RS10160	2192121..2192636	+	516	WP_003409718.1	flavin reductase family protein	-
JE53_RS10165	2192633..2193694	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JE53_RS10170	2193691..2194461	+	771	WP_003409775.1	SDR family oxidoreductase	-
JE53_RS10175	2194671..2195000	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JE53_RS21325	2194997..2195374	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JE53_RS10185	2195371..2195961	-	591	WP_003409784.1	SEC-C domain-containing protein	-
JE53_RS10190	2196016..2197380	+	1365	WP_003903691.1	HNH endonuclease	-
JE53_RS10195	2197535..2197987	-	453	WP_003899095.1	lipoprotein	-
JE53_RS10200	2198051..2198452	+	402	WP_003409869.1	hypothetical protein	-
JE53_RS10205	2198445..2198627	-	183	WP_003409870.1	hypothetical protein	-
JE53_RS10210	2198741..2199091	-	351	WP_003409871.1	hypothetical protein	-
JE53_RS10215	2199102..2200004	-	903	WP_003899097.1	hypothetical protein	-
JE53_RS10220	2200025..2200216	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T48325 WP_003409778.1 NZ_CP009101:c2195000-2194671 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T48325 NZ_CP009101:c2195000-2194671 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT48325 WP_003409780.1 NZ_CP009101:c2195374-2194997 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT48325 NZ_CP009101:c2195374-2194997 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8