Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2869736..2870373 Replicon chromosome
Accession NZ_CP009100
Organism Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag IZ84_RS13470 Protein ID WP_003413180.1
Coordinates 2869736..2870131 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag IZ84_RS13475 Protein ID WP_003413183.1
Coordinates 2870128..2870373 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IZ84_RS13435 2865140..2866351 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
IZ84_RS13440 2866478..2867137 + 660 WP_038435931.1 LppA family lipoprotein -
IZ84_RS13445 2867134..2867796 + 663 WP_038435932.1 hypothetical protein -
IZ84_RS22220 2867793..2868070 + 278 Protein_2629 type II toxin-antitoxin system VapB family antitoxin -
IZ84_RS13450 2868163..2868576 + 414 WP_031660793.1 PIN domain nuclease -
IZ84_RS13455 2868615..2868872 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
IZ84_RS13460 2868869..2869246 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
IZ84_RS13465 2869262..2869636 - 375 WP_003413177.1 hypothetical protein -
IZ84_RS13470 2869736..2870131 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
IZ84_RS13475 2870128..2870373 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
IZ84_RS13480 2870784..2871203 - 420 WP_003413190.1 A24 family peptidase -
IZ84_RS13485 2871215..2872024 - 810 WP_003413193.1 shikimate dehydrogenase -
IZ84_RS13490 2872021..2873277 - 1257 WP_014585306.1 endolytic transglycosylase MltG -
IZ84_RS13495 2873270..2873782 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T48280 WP_003413180.1 NZ_CP009100:c2870131-2869736 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T48280 NZ_CP009100:c2870131-2869736 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT48280 WP_003413183.1 NZ_CP009100:c2870373-2870128 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT48280 NZ_CP009100:c2870373-2870128 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References