Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2868615..2869246 Replicon chromosome
Accession NZ_CP009100
Organism Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag IZ84_RS13460 Protein ID WP_003413174.1
Coordinates 2868869..2869246 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag IZ84_RS13455 Protein ID WP_003413167.1
Coordinates 2868615..2868872 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IZ84_RS13430 2864530..2864844 + 315 WP_009937839.1 hypothetical protein -
IZ84_RS13435 2865140..2866351 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
IZ84_RS13440 2866478..2867137 + 660 WP_038435931.1 LppA family lipoprotein -
IZ84_RS13445 2867134..2867796 + 663 WP_038435932.1 hypothetical protein -
IZ84_RS22220 2867793..2868070 + 278 Protein_2629 type II toxin-antitoxin system VapB family antitoxin -
IZ84_RS13450 2868163..2868576 + 414 WP_031660793.1 PIN domain nuclease -
IZ84_RS13455 2868615..2868872 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
IZ84_RS13460 2868869..2869246 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
IZ84_RS13465 2869262..2869636 - 375 WP_003413177.1 hypothetical protein -
IZ84_RS13470 2869736..2870131 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
IZ84_RS13475 2870128..2870373 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
IZ84_RS13480 2870784..2871203 - 420 WP_003413190.1 A24 family peptidase -
IZ84_RS13485 2871215..2872024 - 810 WP_003413193.1 shikimate dehydrogenase -
IZ84_RS13490 2872021..2873277 - 1257 WP_014585306.1 endolytic transglycosylase MltG -
IZ84_RS13495 2873270..2873782 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T48279 WP_003413174.1 NZ_CP009100:2868869-2869246 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T48279 NZ_CP009100:2868869-2869246 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTACTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT48279 WP_003413167.1 NZ_CP009100:2868615-2868872 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT48279 NZ_CP009100:2868615-2868872 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References