Detailed information of TA system TA048278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2868163..2868872
• Replicon: chromosome
• Accession: NZ_CP009100
• Organism: Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IZ84_RS13450
• Protein ID: WP_031660793.1
• Coordinates: 2868163..2868576 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: IZ84_RS13455
• Protein ID: WP_003413167.1
• Coordinates: 2868615..2868872 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IZ84_RS13425	2863217..2864422	-	1206	WP_003413027.1	chorismate synthase	-
IZ84_RS13430	2864530..2864844	+	315	WP_009937839.1	hypothetical protein	-
IZ84_RS13435	2865140..2866351	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
IZ84_RS13440	2866478..2867137	+	660	WP_038435931.1	LppA family lipoprotein	-
IZ84_RS13445	2867134..2867796	+	663	WP_038435932.1	hypothetical protein	-
IZ84_RS22220	2867793..2868070	+	278	Protein_2629	type II toxin-antitoxin system VapB family antitoxin	-
IZ84_RS13450	2868163..2868576	+	414	WP_031660793.1	PIN domain nuclease	Toxin
IZ84_RS13455	2868615..2868872	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
IZ84_RS13460	2868869..2869246	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
IZ84_RS13465	2869262..2869636	-	375	WP_003413177.1	hypothetical protein	-
IZ84_RS13470	2869736..2870131	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
IZ84_RS13475	2870128..2870373	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
IZ84_RS13480	2870784..2871203	-	420	WP_003413190.1	A24 family peptidase	-
IZ84_RS13485	2871215..2872024	-	810	WP_003413193.1	shikimate dehydrogenase	-
IZ84_RS13490	2872021..2873277	-	1257	WP_014585306.1	endolytic transglycosylase MltG	-
IZ84_RS13495	2873270..2873782	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15018.92 Da
• Isoelectric Point: 5.4673

>T48278 WP_031660793.1 NZ_CP009100:2868163-2868576 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWSAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T48278 NZ_CP009100:2868163-2868576 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTCGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT48278 WP_003413167.1 NZ_CP009100:2868615-2868872 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT48278 NZ_CP009100:2868615-2868872 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829C9W5