Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2234302..2234888 Replicon chromosome
Accession NZ_CP009100
Organism Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag IZ84_RS10445 Protein ID WP_003912702.1
Coordinates 2234302..2234646 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag IZ84_RS10450 Protein ID WP_003410014.1
Coordinates 2234640..2234888 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IZ84_RS10410 2230008..2230607 + 600 WP_003409989.1 L-lysine exporter -
IZ84_RS10415 2231023..2231451 + 429 WP_003409992.1 cellulose-binding protein -
IZ84_RS10420 2231677..2232216 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
IZ84_RS10425 2232736..2233296 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
IZ84_RS10430 2233293..2233634 - 342 WP_003410003.1 DUF2384 domain-containing protein -
IZ84_RS10440 2233830..2234213 - 384 WP_052396876.1 dehydrogenase -
IZ84_RS10445 2234302..2234646 - 345 WP_003912702.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
IZ84_RS10450 2234640..2234888 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
IZ84_RS10455 2234988..2237303 - 2316 WP_003899120.1 cation transporter ATPase CptG -
IZ84_RS10460 2237300..2237572 - 273 WP_003410017.1 DUF1490 family protein -
IZ84_RS10465 2237625..2237981 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
IZ84_RS10470 2238138..2238906 + 769 Protein_2057 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12260.15 Da        Isoelectric Point: 9.8887

>T48270 WP_003912702.1 NZ_CP009100:c2234646-2234302 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPSNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T48270 NZ_CP009100:c2234646-2234302 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCAGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT48270 WP_003410014.1 NZ_CP009100:c2234888-2234640 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT48270 NZ_CP009100:c2234888-2234640 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References