Detailed information of TA system TA048265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194640..2195343
• Replicon: chromosome
• Accession: NZ_CP009100
• Organism: Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: IZ84_RS10165
• Protein ID: WP_003409778.1
• Coordinates: 2194640..2194969 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: IZ84_RS21315
• Protein ID: WP_003409780.1
• Coordinates: 2194966..2195343 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IZ84_RS10145	2191023..2192093	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
IZ84_RS10150	2192090..2192605	+	516	WP_003409718.1	flavin reductase family protein	-
IZ84_RS10155	2192602..2193663	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
IZ84_RS10160	2193660..2194430	+	771	WP_038435902.1	SDR family oxidoreductase	-
IZ84_RS10165	2194640..2194969	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IZ84_RS21315	2194966..2195343	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
IZ84_RS10175	2195340..2195930	-	591	WP_003409784.1	SEC-C domain-containing protein	-
IZ84_RS10180	2195985..2197349	+	1365	WP_003903691.1	HNH endonuclease	-
IZ84_RS10185	2197504..2197956	-	453	WP_003899095.1	lipoprotein	-
IZ84_RS10190	2198020..2198421	+	402	WP_003409869.1	hypothetical protein	-
IZ84_RS10195	2198414..2198596	-	183	WP_003409870.1	hypothetical protein	-
IZ84_RS10200	2198710..2199060	-	351	WP_003409871.1	hypothetical protein	-
IZ84_RS10205	2199071..2199973	-	903	WP_003899097.1	hypothetical protein	-
IZ84_RS10210	2199994..2200185	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T48265 WP_003409778.1 NZ_CP009100:c2194969-2194640 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T48265 NZ_CP009100:c2194969-2194640 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT48265 WP_003409780.1 NZ_CP009100:c2195343-2194966 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT48265 NZ_CP009100:c2195343-2194966 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8