Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1947019..1947632 Replicon chromosome
Accession NZ_CP009100
Organism Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag IZ84_RS09055 Protein ID WP_003408465.1
Coordinates 1947019..1947408 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag IZ84_RS09060 Protein ID WP_003408469.1
Coordinates 1947405..1947632 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IZ84_RS09020 1942648..1943562 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
IZ84_RS09025 1943565..1944395 + 831 WP_003408448.1 cyclase family protein -
IZ84_RS09030 1944395..1944745 + 351 WP_003898986.1 cupin domain-containing protein -
IZ84_RS09035 1944798..1945616 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
IZ84_RS09040 1945630..1946409 + 780 WP_003408460.1 IclR family transcriptional regulator -
IZ84_RS09055 1947019..1947408 - 390 WP_003408465.1 PIN domain-containing protein Toxin
IZ84_RS09060 1947405..1947632 - 228 WP_003408469.1 antitoxin Antitoxin
IZ84_RS09065 1947850..1949334 + 1485 WP_003408473.1 carboxylase -
IZ84_RS09070 1949331..1950578 + 1248 WP_003408476.1 serine hydrolase -
IZ84_RS09075 1950621..1951040 - 420 WP_003408483.1 hypothetical protein -
IZ84_RS09080 1951030..1951740 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T48263 WP_003408465.1 NZ_CP009100:c1947408-1947019 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T48263 NZ_CP009100:c1947408-1947019 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT48263 WP_003408469.1 NZ_CP009100:c1947632-1947405 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT48263 NZ_CP009100:c1947632-1947405 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References