Detailed information of TA system TA048256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239382..1239950
• Replicon: chromosome
• Accession: NZ_CP009100
• Organism: Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: IZ84_RS05890
• Protein ID: WP_003405865.1
• Coordinates: 1239576..1239950 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: IZ84_RS05885
• Protein ID: WP_003405863.1
• Coordinates: 1239382..1239579 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IZ84_RS05865	1235423..1236061	-	639	WP_003898730.1	lipid droplet-associated protein	-
IZ84_RS05870	1236151..1237158	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
IZ84_RS05875	1237175..1238158	-	984	WP_003898731.1	hypothetical protein	-
IZ84_RS05880	1238221..1239294	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
IZ84_RS05885	1239382..1239579	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IZ84_RS05890	1239576..1239950	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
IZ84_RS05895	1240153..1240851	+	699	WP_003898733.1	hypothetical protein	-
IZ84_RS05900	1240969..1241154	+	186	WP_003901093.1	hypothetical protein	-
IZ84_RS05905	1241081..1241356	-	276	WP_038435858.1	hypothetical protein	-
IZ84_RS05910	1241599..1241922	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
IZ84_RS05915	1241937..1242647	-	711	WP_003911399.1	hypothetical protein	-
IZ84_RS22645	1242669..1242878	+	210	WP_003911400.1	hypothetical protein	-
IZ84_RS05925	1242830..1243537	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T48256 WP_003405865.1 NZ_CP009100:1239576-1239950 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T48256 NZ_CP009100:1239576-1239950 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT48256 WP_003405863.1 NZ_CP009100:1239382-1239579 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT48256 NZ_CP009100:1239382-1239579 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07