Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 19722..20244 Replicon plasmid unnamed_2
Accession NZ_CP009074
Organism Escherichia coli ATCC 25922

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag DR76_RS25875 Protein ID WP_000220560.1
Coordinates 19722..20003 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag DR76_RS25880 Protein ID WP_000121743.1
Coordinates 19993..20244 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR76_RS25835 15827..16291 - 465 WP_001443073.1 hypothetical protein -
DR76_RS25840 16548..16934 - 387 WP_000273881.1 hypothetical protein -
DR76_RS25845 17010..17363 - 354 Protein_16 H-NS histone family protein -
DR76_RS28130 17594..17866 - 273 WP_000160399.1 hypothetical protein -
DR76_RS29785 17879..18028 - 150 WP_000003880.1 hypothetical protein -
DR76_RS28135 18098..18293 - 196 Protein_19 hypothetical protein -
DR76_RS25855 18336..18665 - 330 WP_000866648.1 hypothetical protein -
DR76_RS25860 18758..18973 - 216 WP_001180116.1 hypothetical protein -
DR76_RS25865 18963..19208 - 246 WP_000356548.1 hypothetical protein -
DR76_RS25870 19253..19576 - 324 WP_001181903.1 hypothetical protein -
DR76_RS25875 19722..20003 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DR76_RS25880 19993..20244 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
DR76_RS25885 21466..22305 + 840 WP_001546350.1 replication initiation protein -
DR76_RS25890 22326..23168 + 843 WP_000435063.1 replication initiation protein -
DR76_RS25895 23172..23615 + 444 WP_001074379.1 hypothetical protein -
DR76_RS25900 23686..24111 + 426 WP_000202958.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..24185 24185


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T48234 WP_000220560.1 NZ_CP009074:c20003-19722 [Escherichia coli ATCC 25922]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T48234 NZ_CP009074:c20003-19722 [Escherichia coli ATCC 25922]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATTA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT48234 WP_000121743.1 NZ_CP009074:c20244-19993 [Escherichia coli ATCC 25922]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT48234 NZ_CP009074:c20244-19993 [Escherichia coli ATCC 25922]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References