48230

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4733910..4734528 Replicon chromosome
Accession NZ_CP009072
Organism Escherichia coli ATCC 25922

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DR76_RS23575 Protein ID WP_001291435.1
Coordinates 4734310..4734528 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DR76_RS23570 Protein ID WP_000344800.1
Coordinates 4733910..4734284 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR76_RS23560 4729000..4730193 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DR76_RS23565 4730216..4733365 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
DR76_RS23570 4733910..4734284 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DR76_RS23575 4734310..4734528 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DR76_RS23580 4734701..4735252 + 552 WP_000102539.1 maltose O-acetyltransferase -
DR76_RS23585 4735368..4735838 + 471 WP_001304825.1 YlaC family protein -
DR76_RS23590 4736002..4737552 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DR76_RS23595 4737594..4737947 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DR76_RS23600 4738326..4738637 + 312 WP_000409908.1 MGMT family protein -
DR76_RS23605 4738668..4739240 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T48230 WP_001291435.1 NZ_CP009072:4734310-4734528 [Escherichia coli ATCC 25922]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T48230 NZ_CP009072:4734310-4734528 [Escherichia coli ATCC 25922]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT48230 WP_000344800.1 NZ_CP009072:4733910-4734284 [Escherichia coli ATCC 25922]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT48230 NZ_CP009072:4733910-4734284 [Escherichia coli ATCC 25922]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References