Detailed information of TA system TA048229

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4088532..4089366
• Replicon: chromosome
• Accession: NZ_CP009072
• Organism: Escherichia coli ATCC 25922

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: DR76_RS20435
• Protein ID: WP_000854690.1
• Coordinates: 4088532..4088909 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: DR76_RS20440
• Protein ID: WP_001546171.1
• Coordinates: 4088998..4089366 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR76_RS20400	4084123..4084677	-	555	WP_001001909.1	molecular chaperone YcdY	-
DR76_RS20405	4084701..4085438	-	738	WP_000283664.1	zinc-binding phosphatase	-
DR76_RS20410	4085493..4086431	-	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
DR76_RS20420	4086918..4087745	-	828	WP_001304477.1	DUF4942 domain-containing protein	-
DR76_RS20425	4087830..4088027	-	198	WP_000772027.1	DUF957 domain-containing protein	-
DR76_RS20430	4088047..4088535	-	489	WP_001546173.1	hypothetical protein	-
DR76_RS20435	4088532..4088909	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
DR76_RS20440	4088998..4089366	-	369	WP_001546171.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DR76_RS20445	4089416..4090060	-	645	WP_000094915.1	hypothetical protein	-
DR76_RS20450	4090079..4090300	-	222	WP_000692329.1	DUF987 domain-containing protein	-
DR76_RS20455	4090363..4090839	-	477	WP_001537421.1	RadC family protein	-
DR76_RS20460	4090855..4091328	-	474	WP_000855076.1	antirestriction protein	-
DR76_RS20465	4091591..4092412	-	822	WP_001234710.1	DUF945 domain-containing protein	-
DR76_RS29715	4092493..4092704	+	212	Protein_3840	hypothetical protein	-
DR76_RS20475	4092823..4093278	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T48229 WP_000854690.1 NZ_CP009072:c4088909-4088532 [Escherichia coli ATCC 25922]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T48229 NZ_CP009072:c4088909-4088532 [Escherichia coli ATCC 25922]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13745.37 Da
• Isoelectric Point: 4.6220

>AT48229 WP_001546171.1 NZ_CP009072:c4089366-4088998 [Escherichia coli ATCC 25922]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT48229 NZ_CP009072:c4089366-4088998 [Escherichia coli ATCC 25922]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5