Detailed information of TA system TA048228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3994172..3994643
• Replicon: chromosome
• Accession: NZ_CP009072
• Organism: Escherichia coli ATCC 25922

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: DR76_RS19890
• Protein ID: WP_001303511.1
• Coordinates: 3994365..3994643 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: DR76_RS19885
• Protein ID: WP_001302048.1
• Coordinates: 3994172..3994363 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR76_RS19840	3989421..3989639	-	219	WP_000209148.1	DUF4014 family protein	-
DR76_RS19845	3989672..3989884	-	213	WP_000072553.1	hypothetical protein	-
DR76_RS19850	3989990..3990412	-	423	Protein_3723	DUF977 family protein	-
DR76_RS19855	3990428..3991198	-	771	WP_000450998.1	DUF1627 domain-containing protein	-
DR76_RS19860	3991220..3991966	-	747	WP_000788950.1	ATP-binding protein	-
DR76_RS19865	3991973..3992935	-	963	WP_000095673.1	helix-turn-helix domain-containing protein	-
DR76_RS19870	3992958..3993383	-	426	WP_000693943.1	Rha family transcriptional regulator	-
DR76_RS19875	3993380..3993682	-	303	WP_001556930.1	transcriptional regulator	-
DR76_RS28615	3993780..3994151	+	372	WP_001169687.1	hypothetical protein	-
DR76_RS19885	3994172..3994363	+	192	WP_001302048.1	hypothetical protein	Antitoxin
DR76_RS19890	3994365..3994643	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DR76_RS19895	3994935..3995087	+	153	WP_000380313.1	DUF1391 family protein	-
DR76_RS19900	3995099..3995437	+	339	WP_000394541.1	hypothetical protein	-
DR76_RS19905	3995426..3995692	-	267	WP_012601410.1	hypothetical protein	-
DR76_RS19915	3996196..3996384	+	189	WP_000449175.1	cell division inhibitor	-
DR76_RS19920	3996381..3996569	+	189	WP_000199475.1	DUF1482 family protein	-
DR76_RS19925	3996662..3999100	+	2439	WP_000102132.1	exonuclease	-
DR76_RS19930	3999162..3999431	+	270	WP_000003742.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3976776..4003826	27050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T48228 WP_001303511.1 NZ_CP009072:3994365-3994643 [Escherichia coli ATCC 25922]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T48228 NZ_CP009072:3994365-3994643 [Escherichia coli ATCC 25922]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT48228 WP_001302048.1 NZ_CP009072:3994172-3994363 [Escherichia coli ATCC 25922]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT48228 NZ_CP009072:3994172-3994363 [Escherichia coli ATCC 25922]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0