Detailed information of TA system TA048211

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2539247..2540082
• Replicon: chromosome
• Accession: NZ_CP009072
• Organism: Escherichia coli ATCC 25922

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: DR76_RS12335
• Protein ID: WP_000854759.1
• Coordinates: 2539705..2540082 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: DR76_RS12330
• Protein ID: WP_001295723.1
• Coordinates: 2539247..2539615 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR76_RS12295	2536362..2536520	+	159	WP_001323397.1	DUF905 family protein	-
DR76_RS12305	2536675..2537493	+	819	WP_001234738.1	DUF945 domain-containing protein	-
DR76_RS12310	2537835..2538308	+	474	WP_001350782.1	antirestriction protein	-
DR76_RS12315	2538324..2538800	+	477	WP_001186775.1	RadC family protein	-
DR76_RS12320	2538863..2539084	+	222	WP_000692345.1	DUF987 domain-containing protein	-
DR76_RS12330	2539247..2539615	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DR76_RS12335	2539705..2540082	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
DR76_RS12340	2540079..2540567	+	489	WP_000761690.1	hypothetical protein	-
DR76_RS12345	2540584..2540760	+	177	WP_000839286.1	DUF957 domain-containing protein	-
DR76_RS29485	2540860..2541018	+	159	WP_001467148.1	hypothetical protein	-
DR76_RS29045	2541382..2541558	+	177	Protein_2312	helix-turn-helix domain-containing protein	-
DR76_RS12355	2542331..2543971	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2525554..2552981	27427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T48211 WP_000854759.1 NZ_CP009072:2539705-2540082 [Escherichia coli ATCC 25922]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T48211 NZ_CP009072:2539705-2540082 [Escherichia coli ATCC 25922]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT48211 WP_001295723.1 NZ_CP009072:2539247-2539615 [Escherichia coli ATCC 25922]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT48211 NZ_CP009072:2539247-2539615 [Escherichia coli ATCC 25922]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52