Detailed information of TA system TA048205

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 895475..896266
• Replicon: chromosome
• Accession: NZ_CP009072
• Organism: Escherichia coli ATCC 25922

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: DR76_RS04210
• Protein ID: WP_000691791.1
• Coordinates: 895475..895900 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: DR76_RS04215
• Protein ID: WP_000939437.1
• Coordinates: 895931..896266 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR76_RS04180	891141..891638	-	498	WP_000509808.1	hypothetical protein	-
DR76_RS04190	892320..892706	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
DR76_RS04200	893329..894060	-	732	WP_001216639.1	TIGR02646 family protein	-
DR76_RS28875	894057..894278	-	222	WP_125112077.1	hypothetical protein	-
DR76_RS04205	894263..895454	-	1192	Protein_777	AAA family ATPase	-
DR76_RS04210	895475..895900	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
DR76_RS04215	895931..896266	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DR76_RS04220	896266..896739	-	474	WP_001292742.1	DNA repair protein RadC	-
DR76_RS04225	896769..897583	-	815	Protein_781	DUF945 domain-containing protein	-
DR76_RS04230	897819..898772	-	954	WP_000290406.1	hypothetical protein	-
DR76_RS04235	899365..899979	+	615	WP_000772910.1	inovirus Gp2 family protein	-
DR76_RS04240	900097..900315	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	893329..917260	23931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T48205 WP_000691791.1 NZ_CP009072:c895900-895475 [Escherichia coli ATCC 25922]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T48205 NZ_CP009072:c895900-895475 [Escherichia coli ATCC 25922]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT48205 WP_000939437.1 NZ_CP009072:c896266-895931 [Escherichia coli ATCC 25922]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT48205 NZ_CP009072:c896266-895931 [Escherichia coli ATCC 25922]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6