Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 72514..73039 Replicon plasmid p15648-3
Accession NZ_CP009053
Organism Escherichia coli NCCP15648

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag A610_RS29910 Protein ID WP_001159871.1
Coordinates 72514..72819 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A610_RS29915 Protein ID WP_000813634.1
Coordinates 72821..73039 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A610_RS29875 67713..68186 + 474 Protein_91 IS3 family transposase -
A610_RS29880 68197..69024 + 828 Protein_92 translesion error-prone DNA polymerase V subunit UmuC -
A610_RS29885 69056..69448 - 393 WP_000340832.1 plasmid partitioning/stability family protein -
A610_RS29890 69453..70424 - 972 WP_001103689.1 hypothetical protein -
A610_RS29895 70653..71297 + 645 WP_000633911.1 AAA family ATPase -
A610_RS29900 71291..71566 + 276 WP_000239529.1 hypothetical protein -
A610_RS29905 71704..72513 - 810 WP_000016979.1 site-specific integrase -
A610_RS29910 72514..72819 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
A610_RS29915 72821..73039 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A610_RS29920 73401..73589 - 189 Protein_100 IS66 family transposase -
A610_RS29925 73650..74783 + 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
A610_RS29930 75037..76461 - 1425 Protein_102 IS66-like element ISEc23 family transposase -
A610_RS29935 76492..76842 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
A610_RS29940 76839..77264 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B pic / aap/aspU / agg3A / agg3B / agg3C / agg3D 1..78111 78111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T48203 WP_001159871.1 NZ_CP009053:c72819-72514 [Escherichia coli NCCP15648]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T48203 NZ_CP009053:c72819-72514 [Escherichia coli NCCP15648]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT48203 WP_000813634.1 NZ_CP009053:c73039-72821 [Escherichia coli NCCP15648]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT48203 NZ_CP009053:c73039-72821 [Escherichia coli NCCP15648]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References