Detailed information of TA system TA048196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 90504..91105
• Replicon: plasmid p15648-1
• Accession: NZ_CP009051
• Organism: Escherichia coli NCCP15648

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A7A7LY66
• Locus tag: A610_RS28735
• Protein ID: WP_001216036.1
• Coordinates: 90725..91105 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A765X875
• Locus tag: A610_RS28730
• Protein ID: WP_032271830.1
• Coordinates: 90504..90725 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A610_RS28680	85733..85969	+	237	Protein_89	DUF551 domain-containing protein	-
A610_RS28685	85966..86226	+	261	WP_109173355.1	eaa protein	-
A610_RS28690	86303..86536	+	234	WP_000517421.1	hypothetical protein	-
A610_RS28695	86715..87008	+	294	WP_000269001.1	hypothetical protein	-
A610_RS28700	87015..87389	+	375	WP_024222336.1	hypothetical protein	-
A610_RS28705	87371..88015	+	645	WP_109173354.1	hypothetical protein	-
A610_RS28710	88012..88374	+	363	WP_001261544.1	hypothetical protein	-
A610_RS28715	89037..89288	+	252	WP_001667237.1	DNA polymerase III subunit theta	-
A610_RS28720	89295..89867	-	573	WP_001133670.1	hypothetical protein	-
A610_RS28725	90042..90431	+	390	WP_000506730.1	DNA repair protein	-
A610_RS28730	90504..90725	+	222	WP_032271830.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A610_RS28735	90725..91105	+	381	WP_001216036.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
A610_RS28740	91110..91289	+	180	WP_001448367.1	hypothetical protein	-
A610_RS28745	91317..92360	+	1044	WP_032313385.1	DUF968 domain-containing protein	-
A610_RS28750	92449..92901	+	453	WP_023352520.1	hypothetical protein	-
A610_RS28755	92987..94180	+	1194	WP_000219618.1	hypothetical protein	-
A610_RS28760	94180..95664	+	1485	WP_000124159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97258	97258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13613.39 Da
• Isoelectric Point: 5.6343

>T48196 WP_001216036.1 NZ_CP009051:90725-91105 [Escherichia coli NCCP15648]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELVDLTVGAATGEISVSTVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T48196 NZ_CP009051:90725-91105 [Escherichia coli NCCP15648]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGACTCCCCTGAACTGGTAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTACTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8161.16 Da
• Isoelectric Point: 5.1106

>AT48196 WP_032271830.1 NZ_CP009051:90504-90725 [Escherichia coli NCCP15648]
MRSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT48196 NZ_CP009051:90504-90725 [Escherichia coli NCCP15648]
ATGCGATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A7A7LY66

Antitoxin

• AlphaFold DB: A0A765X875