Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5009472..5010273 Replicon chromosome
Accession NZ_CP009050
Organism Escherichia coli NCCP15648

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag A610_RS26425 Protein ID WP_001094430.1
Coordinates 5009472..5009849 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag A610_RS26430 Protein ID WP_001285620.1
Coordinates 5009896..5010273 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A610_RS26400 5005556..5007094 - 1539 WP_001187182.1 lysine decarboxylation/transport transcriptional activator CadC -
A610_RS26410 5007843..5008685 - 843 WP_001290187.1 DUF4942 domain-containing protein -
A610_RS26415 5008770..5008967 - 198 WP_000772029.1 DUF957 domain-containing protein -
A610_RS26420 5008987..5009475 - 489 WP_000761715.1 hypothetical protein -
A610_RS26425 5009472..5009849 - 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
A610_RS26430 5009896..5010273 - 378 WP_001285620.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
A610_RS26435 5010353..5010574 - 222 WP_000692350.1 DUF987 domain-containing protein -
A610_RS26440 5010661..5011137 - 477 WP_001387238.1 RadC family protein -
A610_RS26445 5011153..5011626 - 474 WP_000855057.1 antirestriction protein -
A610_RS30290 5011968..5012093 - 126 Protein_4813 DUF945 domain-containing protein -
A610_RS26460 5012150..5012889 + 740 Protein_4814 IS3 family transposase -
A610_RS26470 5013562..5015070 + 1509 WP_001189118.1 group II intron reverse transcriptase/maturase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T48194 WP_001094430.1 NZ_CP009050:c5009849-5009472 [Escherichia coli NCCP15648]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T48194 NZ_CP009050:c5009849-5009472 [Escherichia coli NCCP15648]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT48194 WP_001285620.1 NZ_CP009050:c5010273-5009896 [Escherichia coli NCCP15648]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT48194 NZ_CP009050:c5010273-5009896 [Escherichia coli NCCP15648]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References