Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4997743..4998240 Replicon chromosome
Accession NZ_CP009050
Organism Escherichia coli NCCP15648

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A610_RS26370 Protein ID WP_001173343.1
Coordinates 4998067..4998240 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A610_RS26365 Protein ID WP_000398619.1
Coordinates 4997743..4998039 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A610_RS26340 4994474..4995193 - 720 WP_000611288.1 two-component system response regulator DcuR -
A610_RS26345 4995190..4996821 - 1632 WP_001216486.1 sensor histidine kinase -
A610_RS26350 4997002..4997232 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
A610_RS26355 4997244..4997516 + 273 WP_000405647.1 N-acetyltransferase -
A610_RS26365 4997743..4998039 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A610_RS26370 4998067..4998240 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A610_RS26375 4998359..4999876 - 1518 WP_001295074.1 lysine--tRNA ligase -
A610_RS26380 5000113..5001570 - 1458 WP_000856837.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T48193 WP_001173343.1 NZ_CP009050:4998067-4998240 [Escherichia coli NCCP15648]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T48193 NZ_CP009050:4998067-4998240 [Escherichia coli NCCP15648]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT48193 WP_000398619.1 NZ_CP009050:4997743-4998039 [Escherichia coli NCCP15648]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT48193 NZ_CP009050:4997743-4998039 [Escherichia coli NCCP15648]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References